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Anti -SF3B14 Rabbit pAb

價格: 在線咨詢
貨號:
KT012086
產品規格:
100 μL
Applications:
WB/IHC/IF
Species:
HMR/HMR
購買數量:
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產品詳情 操作說明 注意事項

Product Information


Description

SF3B14 rabbit polyclonal antibody

Protein full name

Splicing factor 3B subunit 6

Synonyms

Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, Sf3b6, Sf3b14

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse SF3B14

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

14 kDa

Observed MW.

14 kDa

Uniprot ID

Q9Y3B4, P59708




Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1:500-1:1000

testis, colon

IHC/IF

Human, Mouse, Rat

1:500-1:2000

brain, colon cancer, heart, liver




Background


This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.




Images


Western blot analysis of SF3B14 (GB111446) at dilution of 1: 500

Immunohistochemistry of paraffin embedded human brain using SF3B14 (GB111446) at dilution of 1: 1000 (200x lens)

Immunohistochemistry of paraffin embedded human colon cancer using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens)

Immunohistochemistry of paraffin embedded mouse liver using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens)

Immunohistochemistry of paraffin embedded rat heart using SF3B14 (GB111446) at dilution of 1: 1000 (300x lens)

Immunofluorescence of paraffin embedded mouse brain using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).


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