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Anti -RB-1 Rabbit pAb
價格：
在線咨詢
貨號：
DRF101235
產品規格：
100 μL
Applications：
IHC/IF
Species：
M/R,M/R
購買數量：
產品詳情
操作說明
注意事項
Product Information
|
Description |
RB-1 rabbit polyclonal |
|
Protein full name |
Retinoblastoma-associated protein |
|
Synonyms |
pRb, Rb, pp105, Rb1 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse RB-1 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
|
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
IHC |
Mouse, Rat |
1: 700-1: 1400 |
brain, kidney, lung, skeletal muscle |
|
IF |
Mouse, Rat |
1: 500-1: 2500 |
brain, kidney, lung, skeletal muscle |
Background
RB1 belongs to the retinoblastoma protein family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1/H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by Smarxa4/Brg1 by recruiting a histone deacetylase complex to the c-FOS promoter. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Images
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Immunohistochemistry analysis of paraffin-embedded mouse brain using RB-1 (GB111111) at dilution of 1: 1400 |
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Immunohistochemistry analysis of paraffin-embedded rat brain using RB-1 (GB111111) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat kidney using RB-1 (GB111111) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat lung using RB-1 (GB111111) at dilution of 1: 1400 |
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Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using RB-1 (GB111111) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded mouse brain using RB-1 (GB111111) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded rat brain using RB-1 (GB111111) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded rat kidney using RB-1 (GB111111) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraffin-embedded rat lung using RB-1 (GB111111) at dilution of 1: 1000 |
|
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Immunofluorescent analysis of paraffin-embedded mouse skeletal muscle using RB-1 (GB111111) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區黃山路602號合肥國家大學科技園A401室