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400-1016-218
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- 手機：400-1016-218
- Q Q：355185756
- 郵箱：355185756@qq.com
- 地址：安徽省合肥市高新區黃山路602號合肥國家大學科技園A401室
產品詳情
操作說明
注意事項
Product Information
|
Description |
GAPDH mouse monoclonal |
|
Protein full name |
Glyceraldehyde-3-phosphate dehydrogenase |
|
Synonyms |
GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse GAPDH |
|
Isotype |
IgG |
|
Predicted MW. |
37 kDa |
|
Observed MW. |
37 kDa |
|
Subcellular location |
|
|
Purity |
Affinity purification |
|
Uniprot ID |
Applications
|
applications |
species |
dilution |
Positive tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
spleen, lung, kidney, brain, heart, skeletal muscle |
|
IHC |
Human, Mouse, Rat |
1: 200-1: 400 |
stomach, testis, kidney |
|
IF |
Human, Mouse, Rat |
1: 200-1: 400 |
stomach, testis, kidney |
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Images
|
Western blot analysis of GAPDH (GB12002) at dilution of 1:1000 |
|
|
Western blot analysis of GAPDH (GB12002) at dilution of 1:1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded human stomach using GAPDH (GB12002) at dilution of 1:200 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse testis using GAPDH (GB12002) at dilution of 1:200 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat kidney using GAPDH (GB12002) at dilution of 1:200 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
Publications:
|
|
Title |
Journal |
Pubmed ID |
Application |
|
1 |
Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells. |
Aging (Albany NY) |
33290258 |
WB |
|
2 |
Hyperoside Attenuates Bleomycin-Induced Pulmonary Fibrosis Development in Mice. |
Frontiers in Pharmacology |
33192501 |
WB |
|
3 |
African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway. |
Virologica Sinica |
33141406 |
WB |
|
4 |
Bioinformatics analysis of LINC01554 and its co?expressed genes in hepatocellular carcinoma. |
Oncology Reports |
33000250 |
WB |
|
5 |
DEPDC1B promotes migration and invasion in pancreatic ductal adenocarcinoma by activating the Akt/GSK3β/Snail pathway. |
Oncology Letters |
32934714 |
WB |
|
6 |
The tissue specific regulation of miR22 expression in the lung and brain by ribosomal protein L29. |
Scientific Reports |
33004906 |
WB |
|
7 |
Protective effect of dimethyl fumarate on oxidative damage and signaling in cardiomyocytes. |
Molecular Medicine Reports |
32945364 |
WB |
|
8 |
RNF181 modulates Hippo signaling and triple negative breast cancer progression. |
Cancer Cell International |
32655323 |
WB |
|
9 |
A protein palmitoylation cascade regulates microtubule cytoskeleton integrity in Plasmodium. |
The EMBO Journal |
32395856 |
WB |
|
10 |
Hypoxia/reoxygenation activates the JNK pathway and accelerates synovial senescence. |
Molecular Medicine Reports |
32377698 |
WB |
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合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區黃山路602號合肥國家大學科技園A401室