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產品詳情
操作說明
注意事項
Product Information
|
Description |
Lck rabbit polyclonal |
|
Protein full name |
Proto-oncogene tyrosine-protein kinase LCK |
|
Synonyms |
Leukocyte C-terminal Src kinase, |
|
Immunogen |
Recombinant protein corresponding to Mouse Lck |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
58 kDa |
|
Observed MW. |
58 kDa |
|
Subcellular location |
|
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
thymus, lymph nodes, spleen |
|
IHC |
Mouse, Rat |
1: 1500-1: 4000 |
lung, lymph, spleen, thymus |
|
IF |
Mouse, Rat |
1: 1500-1: 3000 |
lung, lymph, spleen |
Background
LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.
Images
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Western blot analysis of Lck (GB111091) at dilution of 1: 1000 Lane 1: Jurkat cell lysate Lane 2: Mouse thymus tissue lysate Lane 3: Mouse lymph nodes tissue lysate Lane 4: Mouse spleen tissue lysate Lane 5: Rat thymus tissue lysate Lane 6: Rat lymph nodes tissue lysate Lane 7: Rat spleen tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900 |
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Immunohistochemistry analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900 |
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Immunohistochemistry analysis of paraffin-embedded rat lymph using Lck (GB111091) at dilution of 1: 1900 |
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Immunohistochemistry analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 1900 |
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Immunohistochemistry analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 1900 |
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Immunohistochemistry analysis of paraffin-embedded mouse thymus using Lck (GB111091) at dilution of 1: 1900 |
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Immunohistochemistry analysis of paraffin-embedded rat thymus using Lck (GB111091) at dilution of 1: 1900 |
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Immunofluorescent analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900 |
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Immunofluorescent analysis of paraffin-embedded rat lung using Lck (GB111091) at dilution of 1: 1900 |
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Immunofluorescent analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900 |
|
|
Immunofluorescent analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 3000 |
|
|
Immunofluorescent analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 3000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區黃山路602號合肥國家大學科技園A401室