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Anti -Hsp47 Rabbit pAb

價格: 在線咨詢
貨號:
KT012065
產(chǎn)品規(guī)格:
100 μL
Applications:
IHC/IF
Species:
H,M,R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

Hsp47 rabbit polyclonal antibody

Protein full name

Serpin H1

Synonyms

47 kDa heat shock protein, Collagen-binding protein, Colligin, Serine protease inhibitor J6, Serpinh1, Cbp1, Hsp47

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Hsp47

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Uniprot ID

P50454, P19324




Applications


Applications

Species

Dilution

Positive Sample

IHC/IF

Human, Mouse, Rat

1: 500-1: 3000

colon cancer, myeloma, ovarian cancer, placenta, skin




Background


Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion.




Images


Immunohistochemistry of paraffin embedded human colon cancer using HSP47 (GB111425) at dilution of 1:1600 (400x lens)

Immunohistochemistry of paraffin embedded human ovarian cancer using HSP47 (GB111425) at dilution of 1:1600 (400x lens)

Immunohistochemistry of paraffin embedded mouse myeloma using HSP47 (GB111425) at dilution of 1:1600 (400x lens)

Immunohistochemistry of paraffin embedded mouse skin using HSP47 (GB111425) at dilution of 1:1600 (400x lens)

Immunohistochemistry of paraffin embedded rat skin using HSP47 (GB111425) at dilution of 1:1600 (400x lens)

Immunofluorescence of paraffin embedded human colon cancer using HSP47 (GB111425) at dilution of 1:800 (400x lens)

Immunofluorescence of paraffin embedded mouse placenta using HSP47 (GB111425) at dilution of 1:800 (400x lens)

Immunofluorescence of paraffin embedded mouse skin using HSP47 (GB111425) at dilution of 1:800 (400x lens)

Immunofluorescence of paraffin embedded rat placenta using HSP47 (GB111425) at dilution of 1:800 (400x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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