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Anti -EEF2/Elongation factor 2 Rabbit pAb

價格: 在線咨詢
貨號:
KT012105
產品規格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,M/R,M/R
購買數量:
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產品詳情 操作說明 注意事項

Product Information


Description

EEF2 rabbit polyclonal

Protein full name

Elongation factor 2

Synonyms

EF-2, Eef2, Eukaryotic translation elongation factor 2; Eukaryotic Elongation Factor-2

Immunogen

Recombinant protein corresponding to Mouse EEF2

Isotype

IgG

Purity

Affinity purification

Predicted MW.

95 kDa

Observed MW.

95 kDa

Subcellular location

Cytoplasm, Nucleus

Uniprot ID

P13639, P58252, P05197




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, pancreas, ovary

IHC

Mouse, Rat

1: 1000-1: 4000

lung, thymus

IF

Mouse, Rat

1: 500-1: 3000

lung, thymus




Background


This gene encodes a member of the GTP-binding translation elongation factor family. This protein catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.




Images


Western blot analysis of EEF2 (GB111099) at dilution of 1: 1000
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Mouse pancreas tissue lysate
Lane 6: Mouse ovary tissue lysate

Immunohistochemistry analysis of paraffin-embedded rat lung using EEF2 (GB111099) at dilution of 1: 700

Immunohistochemistry analysis of paraffin-embedded rat thymus using EEF2 (GB111099) at dilution of 1: 700




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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